phospho smad 1  (Cell Signaling Technology Inc)

Journal: Dentistry Journal

Article Title: Bone Morphogenetic Protein 7 Promotes the Differentiation of Periodontal Ligament Fibroblasts into F-Spondin-Expressing Cementoblast-like Cells During Root Canal Treatment—An In Vivo Rat Pulpectomy Model and In Vitro Human Fibroblast Study

doi: 10.3390/dj13110494

Figure Lengend Snippet: Protein expression levels in the BMP signaling pathway of HPLFs and the inhibition of BMP. SMAD-1/5/9 and phospho-SMAD-1/5/9 were qualitatively detected by Western blot analysis. Stimulation of HPLFs with 100 ng/mL BMP-7 resulted in the phosphorylation of SMAD-1/5/9, but the addition of LDN-193189, an inhibitor of BMP signaling, qualitatively decreased the band intensity of phospho-SMAD-1/5/9 as its concentration increased. Representative qualitative results are shown.

Article Snippet: For the BMP signaling pathway, antibodies for total SMAD-1/5/9 (1:1000, ab80255, Abcam), phospho-SMAD-1/5/9 (1:1000, #13820, Cell Signaling Technology, Beverly, MA, USA), and GAPDH (1:10,000) were used.

Techniques: Expressing, Inhibition, Western Blot, Phospho-proteomics, Concentration Assay